Separation of bacterial capsular and lipopolysaccharides by preparative electrophoresis
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منابع مشابه
Continuous preparative gel electrophoresis.
Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
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Preparative polyacrylamide gel electrophoresis has been used in the final stages of purification to prepare horse serum cholinesterase which was at least 9.5% pure, as judged by analytical polyacrylamide gel electrophoresis. The starting material, already purified about 109-fold by the Strelitz method, was purified an additional 89-fold by the procedure. The method involved Sephadex G-ZOO and S...
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A flat-bed apparatus for preparative gel electrophoresis with intermittent elution [l-31 permits the collection simultaneously of anodeand cathode-migrating components of a mixnue. We employed this first for the separation of isoenzymes of lactate dehydrogenase (EC 1.1.1.27) in crude tissue extracts. Enzyme activity was determined by reaction rate observed at 340 nm in the presence of 0.14 mM N...
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A method of preparative electrophoresis is described. A volume of approximately 1 ml. of protein solution can be applied with the cell used. A “marker” solution, applied adjacent to the sample to be separated, indicates the positions of the protein bands during the migration. The procedure has the advantages of a high degree of recovery, simplicity of operation, and utilization of an electropho...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 1996
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/6.4.433